Agarum clathratum Inhibits LPS-induced IL-1β Expression through the Regulation of the JNK/c-Jun Signaling Pathway
한정민, 조성대, 정지훈, 최수정, 신지애, 홍성두, 홍경옥,
소속 상세정보
한정민 ( Han Jung-Min ) - Seoul National University School of Dentistry Department of Oral Pathology
조성대 ( Cho Sung-Dae ) - Seoul National University School of Dentistry Department of Oral Pathology
정지훈 ( Chung Ge-Hoon ) - Seoul National University School of Dentistry Department of Oral Physiology
최수정 ( Choi Su-Jung ) - Seoul National University School of Dentistry Department of Oral Pathology
신지애 ( Shin Ji-Ae ) - Seoul National University School of Dentistry Department of Oral Pathology
홍성두 ( Hong Seong-Doo ) - Seoul National University School of Dentistry Department of Oral Pathology
홍경옥 ( Hong Kyoung-Ok ) - Seoul National University School of Dentistry Department of Oral Pathology
Abstract
Agarum clathratum (A. clathratum) is a marine brown algal species that belongs to the Costariaceae family and has antioxidant and anti-microbial properties. However, the anti-inflammatory effects of A. clathratum and the molecular mechanisms involved have not been determined so far. This study aimed to investigate the anti-inflammatory effects of A. clathratum extracts in THP-1 macrophages stimulated by lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The THP-1 cells were differentiated with 12-O-tetradecanoylphorbol-13-acetate and treated with A. clathratum before LPS stimulation. Cell viability was assessed using the trypan blue exclusion assay. The expression of pro-inflammatory response-associated molecules was evaluated by quantitative real-time polymerase chain reaction and Western blot analysis. A. clathratum treatment inhibited the expression of interleukin-1β in LPS-stimulated THP-1 macrophages without causing any cytotoxicity. The anti-inflammatory effect of A. clathratum resulted in a significant repression of the JNK/c-Jun signaling axis, a key regulator in inflammation responses. This study highlights the possible role of A. clathratum in the inhibition of pro-inflammatory cytokines via suppression of the JNK/c-Jun signaling axis and suggests that A. clathratum could serve as a marine-derived anti-inflammatory agent in periodontitis.
키워드
Agarum clathratum; inflammation; periodontitis; interleukin-1β; c-Jun N-terminal kinases
원문 및 링크아웃 정보
등재저널 정보